[3H]dynorphin a binding and κ selectivity of prodynorphin peptides in rat, guinea-pig and monkey brain
نویسندگان
چکیده
منابع مشابه
[3H]dynorphin A binding and kappa selectivity of prodynorphin peptides in rat, guinea-pig and monkey brain.
We have previously demonstrated that [3H]dynorphin A selectively labels kappa opioid receptors in guinea-pig whole brain. In these current studies, using protection from inactivation by beta-chloronaltrexamine (beta-CNA), we are able to demonstrate that although dynorphin A prefers kappa receptors, it will label mu receptors when kappa receptors are not available, or present in only a small num...
متن کاملSelectivity of ligand binding to opioid receptors in brain membranes from the rat, monkey and guinea pig.
Conditions for the equilibrium binding to opioid receptor of [3H]sufentanil (mu selective), [3H][D-Pen2,D-Pen5]enkephalin (delta selective), and [3H]U69,593 (kappa selective) were established in membranes from rat brain cerebrum, monkey cortex, or guinea pig cerebellum. The selectivity index of various opioid alkaloids and peptides in binding to the mu, delta, or kappa opioid receptors was expr...
متن کامل[3H] dynorphin binding to guinea pig and rat brain.
[3H] Dynorphin can be shown to bind to the brains of both rat and guinea pigs with approximately 50% specific binding. Characterization of the binding in terms of multiple opiate receptor types supports the kappa selectivity of dynorphin in guinea pig. However, in rat brain, a substantial proportion of the [3H] dynorphin binding is displaced by morphine, suggesting a mu as well as kappa compone...
متن کاملExpression of prodynorphin-derived peptides and mRNA in guinea-pig cortex.
The distributions and extent of processing of four prodynorphin-derived peptides (dynorphin A (1-17), dynorphin A (1-8), dynorphin B, and alpha-neoendorphin) were determined in ten regions of the cortex as well as in the striatum of the guinea-pig. There were significant differences between concentrations of these peptides in most cortical regions, with alpha-neoendorphin being several times mo...
متن کاملProdynorphin peptide immunocytochemistry in rhesus monkey brain.
The present study describes the immunocytochemical distribution of peptides derived from the prodynorphin precursor in the brain of the rhesus monkey (Macaca mulatta). Animals were treated with colchicine (intracerebroventricularly) prior to perfusion to enhance the observation of perikaryal immunoreactivity. Using antisera generated against dynorphin A(1-17), dynorphin B(1-13), and prodynorphi...
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ژورنال
عنوان ژورنال: European Journal of Pharmacology
سال: 1986
ISSN: 0014-2999
DOI: 10.1016/0014-2999(86)90256-6